il10rβ  (Santa Cruz Biotechnology)

Journal: PLoS Pathogens

Article Title: Extrafollicular CD4 T cell-derived IL-10 functions rapidly and transiently to support anti- Plasmodium humoral immunity

doi: 10.1371/journal.ppat.1009288

Figure Lengend Snippet: ( A - D ) 2x10 6 WT or Il10 -/- CD4 T cells were adoptively transferred into Tcra -/- mice 1 day prior to infection with 10 6 P . yoelii pRBC. Spleens were harvested on day 18. Representative plots ( A ) and summary data ( B ) of GC B cells and class-switched GC B cells. Representative plots ( C ) and summary data of GC-TFH cells ( D , left) and parasite-lysate specific IgG2b end point titers ( D , right) on day 18 p.i. Data (means ± S.E.M.) in B,D are from n = 9 WT and n = 8 Il10 -/- recipients pooled from 2 independent experiments and analyzed using unpaired, non-parametric Mann-Whitney tests. ( E ) Competitive mixed bone marrow chimeric mice were generated in which B cells were either WT (B WT , n = 7) or specifically lacked Il10rβ (B IL-10R , n = 10). Representative plots (left) and summary data (right) of TFH cells and GC-TFH cells on day 21 p.i. Data (means ± S.E.M.) were analyzed using unpaired, non-parametric Mann-Whitney tests and are representative of 2 independent experiments. ( F ) Representative plots showing IL-10 expression by CXCR5 + and CXCR5 neg conventional (Foxp3 - ) and regulatory (Foxp3 + ) effector CD4 T cells on day 4 p.i. ( G ) Total numbers of each subset in the spleen on day 4 p.i. Data in F,G (means ± S.D.) and were analyzed using unpaired, non-parametric Mann-Whitney tests and are representative of 2 independent experiments (n = 4 mice). ( H-I ) Histological examination of IL-10 production on day 4 p.i. in the spleens of 10BiT mice stained with anti-B220-AF488 (green) anti-CD4-AF647 (blue) and anti-Thy1.1-PE/Dazzle (red). Data in H-I are representative of 4 independent tiled scans acquired from n = 4 10Bit spleens. ( J ) Summary of the proportion (left) and absolute number (right) of Thy1.1 (IL-10) + cells that localized either outside or within B cell follicles quantified from 25 independent scans and analyzed using unpaired, non-parametric Mann-Whitney tests. ( K-M ) 1 x 10 6 10BiT PbTII cells were CTV-labeled and adoptively transferred into C57BL/6 mice 1 day prior to infection with P . yoelii pRBC. Spleens were harvested on day 4. Representative plots ( K ), and summary data of frequency ( L ) and total numbers ( M ) of CXCR5 + and CXCR5 neg IL-10-expressing PbTII cells. Data (means ± S.D.) in L,M are representative of 2 independent experiments (n = 4) and were analyzed using unpaired, non-parametric Mann-Whitney tests. Scale bars in H and I represent 200 (H) and 20 μm (I), respectively. * P ≤ 0.05 *** P ≤ 0.001 **** P ≤ 0.0001.

Article Snippet: C57BL/6J WT, Il10 -/- , Il10rβ /- , μMT, IL10 -eGFP (Vert-X) [ ], Foxp3-RFP (FIR) [ ], and Tcra -/- [ ] mice (6-to-8 weeks old) were purchased from Jackson Laboratories.

Techniques: Infection, MANN-WHITNEY, Generated, Expressing, Staining, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-10 is essential for the generation of germinal center B cell responses and anti- Plasmodium humoral immunity

doi: 10.4049/jimmunol.1601762

Figure Lengend Snippet: (A) Representative dot plots and summary data showing the proportion of GC (CD19+GL7+) B cells among WT and Il10rβ−/− B cells in Plasmodium infected WT:Il10rβ−/− (1:1) chimeric mice on day 10 p.i. Lines connect proportions of GL7+ GC B cells among WT and Il10rβ−/− cells in the same mouse. Data are representative of 2 independent experiments. (B–D) Mixed bone marrow chimeric mice in which B cells were either WT (BWT) or deficient in expression of Il10rβ−/− (BIL-10R−/−) were infected with P. yoelii. Parasite burdens (B), parasite-specific secreted antibody responses (C), representative dot plots and total number of GC B cells (CD19+GL7+Fas+, D). Data in B are pooled from 2 independent experiments. Data in C,D are from day 21 p.i. and represent 2 independent experiments. Data in B-D are mean ± S.E.M. Data in A were analyzed using paired, nonparametric Wilcoxon matched-pairs signed rank test. Data in B were analyzed using multiple t tests and correcting for multiple comparisons using the Holm-Sidak method. **P ≤ 0.01. Data in C,D were analyzed using unpaired, nonparametric Mann-Whitney tests.

Article Snippet: C57BL/6J WT, Il10 −/− , Il10Rβ −/− , μMT, Tbx21 −/− , and Ifnγr1 −/− (8 weeks old) mice were purchased from Jackson Laboratories.

Techniques: Infection, Expressing, MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-10 is essential for the generation of germinal center B cell responses and anti- Plasmodium humoral immunity

doi: 10.4049/jimmunol.1601762

Figure Lengend Snippet: (A–B) WT and Il10−/− mice were infected with P. yoelii. (A) Representative dot plots and summary data depicting the proportion of GC B cells (CD19+GL7+Fas+) expressing Bcl-6 and T-bet on day 10 p.i. (B) Ratio of T-bet+ GC B cells to Bcl-6+ GC B cells. Data are pooled from 3 independent experiments. (C) Proportion of T-bet+ GC B cells (CD19+GL7+) in WT:Il10rβ−/− chimeric mice on day 10 p.i. Lines connect proportions of T-bet+ GC B cells among WT and Il10rβ−/− cells in the same mouse. Data are representative of 2 independent experiments. (D–E) WT and Il10−/− mice were infected with P. yoelii. Il10−/− mice were treated with either 500 µg of rIgG or α-IFN-γ every two days starting on day -1. (D) Representative dot plots and summary data showing the proportion GC B cells (CD19+GL7+Fas+) expressing Bcl-6 and T-bet on day 10 p.i. (E) Ratio of T-bet+ GC B cells to Bcl-6+ GC B cells in mice from each experimental group. Data are pooled from 2 independent experiments. (F–H) WT:Ifnγr1−/− mixed bone marrow chimeric mice were infected with P. yoelii. Representative dot plots and summary data showing the proportion GC B cells (CD19+GL7+) (F), Bcl-6+ GC B cells (G), and T-bet+ GC B cells (H) among WT and Ifnγr1−/− cells recovered on day 10 p.i. Lines connect proportions in the same mice. Data are representative of 2 independent experiments. In C, G, and H, gray histograms represent isotype control staining. Data in A,B,D–E are mean ± S.E.M. Data in A and B were analyzed using unpaired, nonparametric Mann-Whitney tests. Data in C and F–H were analyzed using paired, nonparametric Wilcoxon matched-pairs signed rank tests. Data in D and E were analyzed using unpaired, nonparametric Kruskal-Wallis test correcting for multiple comparisons via Dunn’s tests.

Article Snippet: C57BL/6J WT, Il10 −/− , Il10Rβ −/− , μMT, Tbx21 −/− , and Ifnγr1 −/− (8 weeks old) mice were purchased from Jackson Laboratories.

Techniques: Infection, Expressing, Control, Staining, MANN-WHITNEY